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- Title
Isolation and Purification, Structural Characterization and Antioxidant Activities of a Novel Hetero-Polysaccharide from Steam Exploded Wheat Germ.
- Authors
Hu, Lei; Zhou, Xiaodan; Tian, Xue; Li, Ranran; Sui, Wenjie; Liu, Rui; Wu, Tao; Zhang, Min
- Abstract
A purified polysaccharide, designated as SE-WGPI, was isolated from wheat germ modified by steam explosion. The primary structure characteristics were determined by HPGPC, GC, periodate oxidation-Smith degradation, methylation analysis, FT-IR, NMR and Congo red test. The results showed that SE-WGPI was a homogeneous hetero-polysaccharide with the average molecular weight of 5.6 × 103 Da. The monosaccharide composition mainly consisted of glucose, arabinose and xylose with a molar ratio of 59.51: 20.71: 19.77. The main backbone of SE-WGPI consisted of →4,6)-α-D-Glcp(1→6)-α-D-Glcp(1→3)-β-D-Xylp(1→5)-α-L-Araf(1→ and the side chain was α-D-Glcp(1→ linked at the C4-position of →4,6)-α-D-Glcp(1→. SE-WGPI likely has a complex netted structure with triple helix conformation and good thermal stability. In addition, SE-WGPI had valid in vitro radical scavenging activities on DPPH and hydroxyl radicals. This study may provide structural information of SE-WGPI for its promising application in the fields of functional foods or medicines.
- Subjects
WHEAT germ; CONGO red (Staining dye); MONOSACCHARIDES; HYDROXYL group; MOLECULAR weights; POLYSACCHARIDES; ARABINOSE; FUNCTIONAL foods
- Publication
Foods, 2022, Vol 11, Issue 9, p1245
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods11091245