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- Title
Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae.
- Authors
Dong-Hwan Kim; Harris, Bethany; Fei Wang; Seidel, Chris; McCroskey, Scott; Gerton, Jennifer L.
- Abstract
The budding yeast E3 SUMO ligase Mms21, also known as Nse2, is a component of the Smc5/6 complex, which regulates sister chromatid cohesion, DNA replication, and repair. Our study shows that the mms21RINGD mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; and (4) upregulation of Gcn4 targets. Genes involved in ribosome biogenesis and translation are downregulated in the mms21RINGD mutant. We identified RPL19A as a novel genetic suppressor of the mms21RINGD mutant. Deletion of RPL19A partially suppresses growth defects in both smc5-6 and mms21RINGD mutants as well as nuclear accumulation of ribosome subunits in the mms21RINGD mutant. Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress. This study suggests that the Smc5/6 complex supports nucleolar function.
- Subjects
SACCHAROMYCES cerevisiae; LIGASES; NUCLEAR proteins; BUDDING (Zoology); GENETIC translation; PHYSIOLOGICAL stress; DOWNREGULATION
- Publication
Genetics, 2016, Vol 204, Issue 2, p645
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.115.181750