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- Title
Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila.
- Authors
Zhongsheng Yu; Mengda Ren; Zhanxiang Wang; Bo Zhang; Yikang S. Rong; Renjie Jiao; Guanjun Gao
- Abstract
We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.
- Subjects
DROSOPHILA; HETEROCHROMATIN; EUCHROMATIN; INSECT genetics; GERM cells
- Publication
Genetics, 2013, Vol 195, Issue 1, p289
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.113.153825