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- Title
Tracing the Emergence of a Novel Sex-Determining Gene in Medaka, Oryzias luzonensis.
- Authors
Myosho, Taijun; Otake, Hiroyuki; Masuyama, Haruo; Matsuda, Masaru; Kuroki, Yoko; Fujiyama, Asao; Naruse, Kiyoshi; Hamaguchi, Satoshi; Sakaizumi, Mitsuru
- Abstract
Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of Gsdf contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis.
- Subjects
VERTEBRATES; XENOPUS laevis; MAMMALS; GENETIC engineering; CYTOKINES
- Publication
Genetics, 2012, Vol 191, Issue 1, p163
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.111.137497