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- Title
Protective Role of Putrescine against Picloram Induced Genomic Instability and DNA Methylation in Phaseolus vulgaris.
- Authors
TAŞPINAR, Mahmut Sinan; ARSLAN, Esra; AYDIN, Murat; SIĞMAZ, Burcu; AĞAR, Güleray
- Abstract
Picloram (4-amino-3-5-6-trichloropicolinic acid) is an important synthetic auxin and it is one of the most widely used herbicides in agriculture. The use of picloram is representing a potential hazardous to ecosystems and the human health. Putrescine is a kind of polyamine which has a role in plant metabolism such as protecting membrane stability, removing free radicals and nucleic acid and protein synthesis, etc. In this study, DNA damage levels in Phaseolus vulgaris treated with picloram, DNA methylation changes and whether putrescine was any alleviative impact on these alterations were investigated. DNA methylation pattern changes and DNA damage levels were determined by using Randomly Amplified Polymorphic DNA (RAPDs) and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RAs). The obtained results indicated that all doses of picloram (5, 10, 20 and 40 mg L-1) had a negative effect on RAPDs profile changes (increased DNA damage levels) and decreased of Genomic Template Stability (GTS) and also DNA hypomethylation was seen. However, all concentrations of putrescine (0.01, 0.1 and 1 ppm) reduced these harmful effects of picloram. Consequently, putrescine can be an alternative for reducing genotoxic damage against chemical mutagens in plants.
- Subjects
PUTRESCINE; PICLORAM; DNA methylation; KIDNEY bean; FREE radicals; PROTEIN synthesis
- Publication
Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2017, Vol 7, Issue 4, p271
- ISSN
2146-0574
- Publication type
Article
- DOI
10.21597/jist.2017.221