We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The role of stem cell factor and granulocyte-colony stimulating factor in brain repair during chronic stroke.
- Authors
Chun-Shu Piao; Gonzalez-Toledo, Maria E.; Yue-Qiang Xue; Wei-Ming Duan; Terao, Satoshi; Granger, D. Neil; Kelley, Roger E.; Li-Ru Zhao
- Abstract
Chronic stroke is a highly important but under-investigated scientific problem in neurologic research. We have reported earlier that stem cell factor (SCF) in combination with granulocyte-colony stimulating factor (G-CSF) treatment during chronic stroke improves functional outcomes. Here we have determined the contribution of bone marrow-derived cells in angiogenesis and neurogenesis, which are enhanced by SCF+G-CSF treatment during chronic stroke. Using bone marrow tracking, flow cytometry, 2-photon live brain imaging, and immunohistochemistry, we observed that the levels of circulating bone marrow stem cells (BMSCs) (CD34+/c-kit+) were significantly increased by SCF+G-CSF treatment. In addition, live brain imaging revealed that numerous bone marrow-derived cells migrate into the brain parenchyma in the treated mice. We also found that bone marrow-derived cells, bone marrow-derived endothelial cells, vascular density, and bone marrow-derived neurons were significantly augmented by SCF+G-CSF. It is interesting that, in addition to the increase in bone marrow-derived endothelial cells, the number of bone marrow-derived pericytes was reduced after SCF+G-CSF treatment during chronic stroke. These data suggest that SCF+G-CSF treatment can enhance repair of brain damage during chronic stroke by mobilizing BMSCs, and promoting the contribution of bone marrow-derived cells to angiogenesis and neurogenesis.Journal of Cerebral Blood Flow & Metabolism (2009) 29, 759–770; doi:10.1038/jcbfm.2008.168; published online 11 February 2009
- Subjects
CEREBROVASCULAR disease; NEUROLOGY; STEM cells; GRANULOCYTES; BONE marrow; CELLS
- Publication
Journal of Cerebral Blood Flow & Metabolism, 2009, Vol 29, Issue 4, p759
- ISSN
0271-678X
- Publication type
Article
- DOI
10.1038/jcbfm.2008.168