We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Regeneration characteristics of different dental derived stem cell sheets.
- Authors
Hu, Lei; Zhao, Bin; Gao, Zhenhua; Xu, Junji; Fan, Zhipeng; Zhang, Chunmei; Wang, Jinsong; Wang, Songlin
- Abstract
Background: Although cell sheets have gained much interest as a non‐scaffold strategy for tissue regeneration, the regenerative features of different cell sheets remain unclear. Objective: In this study, we aimed to compare the regeneration characteristics of cell sheets derived from dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs) and stem cells of the apical papilla (SCAPs). Methods: Dental pulp stem cells, PDLSCs and SCAPs from the same individual were acquired and induced to form sheets using 20 μg/mL vitamin C. Immunofluorescence staining was used to detect the expression of collagen I, fibronectin, integrin β1 and vimentin. Real‐time PCR was used to determine NANOG, OCT4, SOX2 and TERT gene expression. The cell sheets with hydroxyapatite/tricalcium phosphate were transplanted into nude mice subcutaneously to evaluate tissue regeneration characteristics. Results: No obvious differences were found in the histological structure and extracellular matrix protein expression between DPSC, PDLSC and SCAP sheets. Dental pulp stem cell sheet showed higher expression of OCT4 and TERT than PDLSC and SCAP sheets. All three cell sheets displayed the ability of mineral tissue formation and highly expressed periostin. The tissue derived from DPSC sheet showed higher CD31 expression and porous fibres compared with that from the others. The tissue fibres formed from PDLSC sheet were directionally arranged, while the tissue derived from SCAP sheet showed highest mineral tissue formation. Conclusion: Although in vitro DPSC, PDLSC and SCAP cell sheets have similar characteristics, their regenerative characteristics in vivo are different, with each showing potential application for regeneration of different tissues.
- Subjects
STEM cell transplantation; ANIMAL experimentation; CELL culture; COLLAGEN; DENTAL pulp; FIBRONECTINS; FLUORESCENT antibody technique; GENE expression; IMMUNOHISTOCHEMISTRY; MICE; PERIODONTAL ligament; POLYMERASE chain reaction; REGENERATION (Biology); STAINS &; staining (Microscopy); VITAMIN C; TISSUE engineering; DATA analysis software; DESCRIPTIVE statistics; ONE-way analysis of variance
- Publication
Journal of Oral Rehabilitation, 2020, Vol 47, p66
- ISSN
0305-182X
- Publication type
Article
- DOI
10.1111/joor.12839