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- Title
Karbon Tetraklorürün Neden Olduğu Akciğer Hasarında L-Karnitinin Antiapoptotik ve Histopatolojik Değişiklikler Üzerindeki Rolü.
- Authors
YILDIRIM, Ayşegül Burçin; KARABULUT, Derya
- Abstract
Background: The aim of this study to investigate the toxic effects of Carbon tetrachloride (CCl4) exposure on the lung, the protective and therapeutic role of L-carnitine against these toxic effects and the change of caspase-3 activity. Materials and Methods: In the study, 25 rats were divided into 5 groups, with 5 in each group. Groups were created as follows. Group I: Control Group: 0.2 ml of olive oil twice a week for 6 weeks Intraperitoneal (ip); Group II: L-carnitine group, 200 mg/kg L-carnitine twice a week for 6 weeks (ip); Group III: CCl4 group: 0.2 ml/100 g CC14 twice a week for 6 weeks (ip); Group IV: CCI4 + L-carnitine group, 200 mg/kg L-carnitine half an hour after applying 0.2 ml/100 g CCl4 twice a week for 6 weeks (ip); Group V: L-carnitine + CCl4 group, 200 mg/kg L-carnitine 24 hours before 0.2 ml/100 g CCl4 application twice a week for 6 weeks (ip). Both CCl4 and L-carnitine administration was continued twice a week for 6 weeks. Lung histopathology was evaluated, and caspase-3 enzyme activity was determined by immunohistochemical method. Caspase-3 immunopositive cells were counted and statistical analysis was performed. Results: An obvious difference was observed in the lung tissue histology of the CCl4 administered group. Histopathological changes such as increase in alveolar interstitial areas, vacuolization and degeneration in bronchiolar epithelium were encountered. According to the results of statistical analysis, Group III and Group V values were significantly higher than the control group. When L-carnitine was given before CCl4, the number of caspase-3 cells was higher than the control group. And when L-carnitine was given later than CCl4, the number of caspase-3 cells was similar with the control group. Conclusion: As a result, we think that L-carnitine supplement has an anti-apoptotic effect and it can use as a therapeutic agent in alveolar damage formation.
- Subjects
ANIMAL experimentation; APOPTOSIS; CARNITINE; HISTOLOGICAL techniques; HYDROCARBONS; LUNGS; RATS; CASPASES
- Publication
Journal of Harran University Medical Faculty / Harran Üniversitesi Tıp Fakültesi Dergisi, 2020, Vol 17, Issue 2, p189
- ISSN
1304-9623
- Publication type
Article
- DOI
10.35440/hutfd.717101