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- Title
Imaging an Adapted Dentoalveolar Complex.
- Authors
Herber, Ralf-Peter; Justine Fong; Lucas, Seth A.; Ho, Sunita P.
- Abstract
Adaptation of a rat dentoalveolar complex was illustrated using various imaging modalities. Micro-X-ray computed tomography for 3D modeling, combined with complementary techniques, including image processing, scanning electron microscopy, fluorochrome labeling, conventional histology (H&E, TRAP), and immunohistochemistry (RANKL, OPN) elucidated the dynamic nature of bone, the periodontal ligament-space, and cementum in the rat periodontium. Tomography and electron microscopy illustrated structural adaptation of calcified tissues at a higher resolution. Ongoing biomineralization was analyzed using fluorochrome labeling, and by evaluating attenuation profiles using virtual sections from 3D tomographies. Osteoclastic distribution as a function of anatomical location was illustrated by combining histology, immunohistochemistry, and tomography. While tomography and SEM provided past resorption-related events, future adaptive changes were deduced by identifying matrix biomolecules using immunohistochemistry. Thus, a dynamic picture of the dentoalveolar complex in rats was illustrated.
- Subjects
MEDICAL imaging systems; ALVEOLAR process; SCANNING electron microscopy; LABORATORY rats; BIOMINERALIZATION; COMPUTED tomography; IMMUNOHISTOCHEMISTRY; FLUOROPHORES
- Publication
Anatomy Research International, 2012, p1
- ISSN
2090-2743
- Publication type
Article
- DOI
10.1155/2012/782571