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- Title
Immobilization of the Gas Signaling Molecule H<sub>2</sub>S by Radioisotopes: Detection, Quantification, and In Vivo Imaging.
- Authors
Sarkar, Swarbhanu; Ha, Yeong Su; Soni, Nisarg; An, Gwang Il; Lee, Woonghee; Kim, Min Hwan; Huynh, Phuong Tu; Ahn, Heesu; Bhatt, Nikunj; Lee, Yong Jin; Kim, Jung Young; Park, Kwon Moo; Ishii, Isao; Kang, Shin-Geol; Yoo, Jeongsoo
- Abstract
Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin-layer radiochromatography method. When 64Cu-cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter-sized infarcted lesion of the rat heart.
- Subjects
ENDOGENOUS hydrogen sulfide; RADIONUCLIDE imaging; ENCAPSULATION (Catalysis); MACROCYCLIC compounds; CHERENKOV radiation; POSITRON emission tomography
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 32, p9365
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201603813