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- Title
Restriction fragment length polymorphism of urease C and urease B genes of Helicobacter pylori strains isolated from Brazilian patients with peptic ulcer and chronic gastritis.
- Authors
Röesler, Bruna Maria; De Oliveira, Telma Barbosa; Bonon, Sandra Delena Alves; Monici, Leonardo Trevisan; Zeitune, José Murilo Robilotta; Costa, Sandra Cecília Botelho; Röesler, Bruna Maria; Bonon, Sandra Helena Alves; Zeitune, José Murilo Robilotta; Costa, Sandra Cecília Botelho
- Abstract
The aim of the present work is to identify the presence of Helicobacter pylori bacterium in samples of gastric mucosa fragments, obtained by gastric biopsy, from Brazilian patients with peptic ulcer and chronic gastritis and also to determine differences among the prevalent strains in these two diseases by urease C and urease B genes amplification utilizing nested polymerase chain reaction (PCR) and PCR. We encountered 17 genotyping patterns for urease C and 7 for urease B and, although no significant differences were found among the patterns encountered for both diseases, we found predominant groups for each disease. Typing methods of the products obtained by nested PCR and PCR show a functional scheme and are of great importance for epidemiologic studies and H. pylori strain characterization, in addition to allowing correlation among the several strains and their role in the diseases caused by this microorganism.
- Subjects
BRAZIL; RESTRICTION fragment length polymorphisms; GENETIC polymorphisms; UREASE; HELICOBACTER pylori; PEPTIC ulcer; STOMACH biopsy; POLYMERASE chain reaction; AGAR; BACTERIAL proteins; BACTERIOPHAGE typing; CARRIER proteins; CHRONIC diseases; COMPARATIVE studies; ELECTROPHORESIS; GASTRIC mucosa; GASTRITIS; GENES; HELICOBACTER diseases; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; GENOTYPES; DISEASE complications
- Publication
Digestive Diseases & Sciences, 2009, Vol 54, Issue 7, p1487
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-008-0525-2