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- Title
Bioassay-guided isolation of active anti-adipogenic compound from royal jelly and the study of possible mechanisms.
- Authors
Pandeya, Prakash Raj; Lamichhane, Ramakanta; Lee, Kyung-Hee; Kim, Se-Gun; Lee, Dae-Ho; Lee, Hyeong-Kyu; Jung, Hyun-Ju
- Abstract
Background: Royal jelly (RJ) has been used traditionally for dietary, cosmetic and health purposes for a long time in different parts of the world. Scientific studies have also shown its numerous health-promoting properties including hypoglycemic and anti-hypercholesterolemic action. In this study, we investigated the anti-adipogenic activity of RJ in 3 T3-L1 cells and isolated the major responsible root component for the activity. Methods: An active anti-adipogenic compound was isolated through bioassay-guided isolation process by successive treatment of RJ and its active fractions on 3 T3-L1 cell line. (E)-10-Hydroxy-2-decenoic Acid (10-HDA) was identified using NMR spectroscopy and ultra-performance liquid chromatography (UPLC). As 10-HDA showed significant anti-adipogenic activity with Oil Red O staining and TG content assay on 3 T3-L1 adipocytes, further study was carried out in molecular level for the expression of adipogenic transcription factors such as PPARγ, FABP4, C/EBPα, SREBP-1c, and Leptin. The effect of 10-HDA on preliminary molecules such as pAkt, pERK, C/EBPβ, and pCREB were studied in the early stage of adipogenesis. The effect of 10-HDA on reactive oxygen species (ROS) production in fully differentiating adipocytes was measured by nitro blue tetrazolium (NBT) assay. Result: Results showed that triacylglycerol accumulation and ROS production was markedly suppressed by 10-HDA. Preliminary molecules such as pAkt, pERK, pCERB, and C/EBPβ were found to be down-regulated by 10-HDA, which led to down-regulation of key adipogenic transcription factors such as PPARγ, FABP4, CEBPα, SREBP-1c, and Leptin on 3 T3-L1 adipocytes. Conclusion: Our results suggest that anti-adipogenesis of 10-HDA on 3 T3-L1 adipocyte takes place via two mechanisms: inhibition of cAMP/PKA pathway and inhibition of p-Akt and MAPK dependent insulin signaling pathway. So it is considered that 10-HDA, a major component of RJ, can be a potential therapeutic medicine for obesity.
- Subjects
REACTIVE oxygen species; BIOLOGICAL assay; CELLULAR signal transduction; FAT cells; FATTY acid-binding proteins; GENE expression; HIGH performance liquid chromatography; NUCLEAR magnetic resonance spectroscopy; OBESITY; STAINS &; staining (Microscopy); TRANSCRIPTION factors; TRIGLYCERIDES; LEPTIN; PEROXISOME proliferator-activated receptors
- Publication
BMC Complementary & Alternative Medicine, 2019, Vol 19, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-018-2423-2