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- Title
Label‐Free Electrochemiluminescence Imaging of Single‐Cell Adhesions by Using Bipolar Nanoelectrode Array.
- Authors
Qin, Xiang; Jin, Hua‐Jiang; Li, Xiuxiu; Li, Jian; Pan, Jian‐Bin; Wang, Kang; Liu, Songqin; Xu, Jing‐Juan; Xia, Xing‐Hua
- Abstract
A label‐free and fast approach for positive electrochemiluminescence (ECL) imaging of single cells by bipolar nanoelectrode array is proposed. The reduction of oxygen at a platinized gold nanoelectrode array in a closed bipolar electrochemical system is coupled with an oxidative ECL process at the anodic side. For elevating the ECL imaging contrast of single cells, a driving voltage of −2.0 V is applied to in situ generate oxygen confined beneath cells that is subsequently used for ECL imaging at 1.1 V. High oxygen concentration in the confined space resulting from steric hindrance generates prominent oxygen reduction current at the cathodic side and higher ECL intensity at the anodic side, allowing positive ECL imaging of the cells adhesion region with excellent contrast. Cell morphology and adhesion strength can be successfully imaged with high image acquisition rate. This approach opens a new avenue for label‐free imaging of single cells.
- Subjects
ELECTROCHEMILUMINESCENCE; BIPOLAR cells; CELL imaging; STERIC hindrance; OXYGEN reduction; TISSUE arrays; CELL adhesion
- Publication
Chemistry - A European Journal, 2022, Vol 28, Issue 3, p1
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.202103964