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- Title
Digital spatial profiling of CD4<sup>+</sup> T cells in classic Hodgkin lymphoma.
- Authors
Takeuchi, Mai; Miyoshi, Hiroaki; Semba, Yuichiro; Yamada, Kyohei; Nakashima, Kazutaka; Sato, Kensaku; Furuta, Takuya; Moritsubo, Mayuko; Ogura, Yusuke; Tanaka, Ken; Imamoto, Teppei; Arakawa, Fumiko; Kohno, Kei; Ohshima, Koichi
- Abstract
Classic Hodgkin lymphoma (CHL) harbors a small number of Hodgkin-Reed-Sternberg (HRS) cells scattered among numerous lymphocytes. HRS cells are surrounded by distinct CD4+ T cells in a rosette-like manner. These CD4+ T cell rosettes play an important role in the tumor microenvironment (TME) of CHL. To elucidate the interaction between HRS cells and CD4+ T cell rosettes, we completed digital spatial profiling to compare the gene expression profiles of CD4+ T cell rosettes and other CD4+ T cells separated from the HRS cells. Immune checkpoint molecules including OX40, programed cell death-1 (PD-1), and cytotoxic T lymphocyte associated protein 4 (CTLA-4) expression was higher in CD4+ T cell rosettes compared to other CD4+ T cells. Immunohistochemistry confirmed variable PD-1, CTLA-4, and OX40 expression in the CD4+ T cell rosettes. This study introduced a new pathological approach to study the CHL TME, and provided deeper insight into CD4+ T cells in CHL.
- Subjects
GENE expression profiling; HODGKIN'S disease; IMMUNE checkpoint proteins; CYTOTOXIC T cells; T cells
- Publication
Virchows Archiv: European Journal of Pathology, 2023, Vol 483, Issue 2, p255
- ISSN
0945-6317
- Publication type
Article
- DOI
10.1007/s00428-023-03562-1