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- Title
Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells.
- Authors
Li, Qi; Wang, Jianliu; Liu, Haifeng; Xie, Bing; Wei, Lihui
- Abstract
A tissue-engineered mesh fabricated with adipose-derived mesenchymal stem cells (AD-MSCs) cultured on a silk fibroin scaffold is evaluated for use in female pelvic reconstruction. Thirty-five female Sprague Dawley rats were divided into four groups. Group A ( n = 10) were implanted with polypropylene meshes, Group B ( n = 10) with silk fibroin scaffolds and Group C ( n = 10) with tissue-engineered meshes. Group D ( n = 5) acted as the tissue control. The tissue-engineered mesh was produced as follows. AD-MSCs were obtained from adipose tissue of rats designated to Group C. The cells were seeded onto a silk fibroin scaffold, cultured and then observed by scanning electron microscopy (SEM). Histological studies of these meshes were performed at 4 and 12 weeks after implantation and mechanical testing was carried out on all groups before implantation and at 12 weeks after implantation. AD-MSCs displayed fibroblast-like shapes and were able to differentiate into adipocytes or fibroblasts. SEM observation showed that AD-MSCs proliferated and secreted a matrix onto the silk fibroin scaffolds. After implantation of the scaffolds into rats, histological analysis revealed better organized newly formed tissue in Group C than in controls. Group C also had a similar failure force (2.67 ± 0.15 vs 2.33 ± 0.38 N) and a higher Young's modulus (2.99 ± 0.19 vs 1.68 ± 0.20 MPa) than a normal vaginal wall, indicating the potential of this tissue-engineered approach. AD-MSCs were validated as seed cells for tissue engineering. The silk fibroin scaffold thus shows promise for application with AD-MSCs in the fabrication of tissue-engineered mesh with good biocompatibility and appropriate mechanical properties for pelvic floor reconstruction.
- Subjects
TISSUE engineering; PELVIC floor; POLYPROPYLENE; SILK fibroin; TISSUE scaffolds; FAT cells; MESENCHYMAL stem cells
- Publication
Cell & Tissue Research, 2013, Vol 354, Issue 2, p471
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-013-1719-2