We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enhancement of volatile thiol release of Saccharomyces cerevisiae strains using molecular breeding.
- Authors
Dufour, Matthieu; Zimmer, Adrien; Thibon, Cécile; Marullo, Philippe
- Abstract
Cysteine-conjugated volatile thiols are powerful aromatic compounds that contribute to the fruity notes of many white wines and especially Sauvignon Blanc. Genetic selection programs of wine yeast starters able to produce more volatile thiols constitute, therefore, an important goal for the wine industry. Recent investigations on yeast metabolism suggested that the ß-lyase Irc7p and the control of its gene expression by nitrogen catabolite repression constitute a rational way for yeast genetic improvement. This work demonstrates that the use of a natural ure2 mutation can be used to design wine starters with an enhanced capacity of volatile thiols production. By applying backcrosses driven by molecular markers, this allelic form was introduced in different starter backgrounds. Our investigations demonstrate that the ure2 inheritance is able to enhance the production of 4MMP (recently renamed 4MSP) and 3MH (recently renamed 3SH). For 4MMP, this effect depends of the presence of the allele IRC7 encoding a long form of the Irc7 protein. Moreover, a correlation in between the expression level of this allelic form and 4MMP production was found within industrial starters. All together, these results emphasised the use of molecular breeding for improving quantitative traits of industrial strains without the use of genetically modifying strategies.
- Subjects
SACCHAROMYCES cerevisiae; THIOLS; VOLATILE organic compounds; MOLECULAR microbiology; BIOMARKERS; YEAST research; MATRILYSIN
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 13, p5893
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-4739-7