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- Title
Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus.
- Authors
Rui Luo; Liurong Fang; Yunbo Jiang; Hui Jin; Yanwei Wang; Dang Wang; Huanchun Chen; Shaobo Xiao
- Abstract
Nuclear factor kappa B (NF-κB) is a critical transcription factor in innate and adaptive immune response as well as cell proliferation and survival. Previous studies have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection activated NF-κB pathways through IκB degradation in MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, we investigated the role of PRRSV structural proteins in the regulation of NF-κB. In this study, we screened the structural proteins of PRRSV by NF-κB DNA-binding assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) protein could activate NF-κB in MARC-145 cells. Furthermore, we revealed that the region between aa 30 and 73 of N protein was essential for its function in the activation of NF-κB. These results presented here provide a basis for understanding molecular mechanism of PRRSV infection and inflammation response.
- Subjects
NF-kappa B; GENITAL diseases; RESPIRATORY diseases; SWINE diseases; CELL proliferation; LUCIFERASES
- Publication
Virus Genes, 2011, Vol 42, Issue 1, p76
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-010-0548-6