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- Title
Oxidative damage to boar sperm by ultraviolet irradiation.
- Authors
Liu, H. Y.; Wang, P. S.; Zeng, Y. W.; Hsu, L. T.; Kuo, H. H.; Hu, N. Y.; Wang, S. C.; Chen, M. C.
- Abstract
Sperm are vulnerable to oxidative damage during the preservation of boar semen. Light is one of the causes of the damage. Therefore, collected semen should be preserved in the dark to reduce the oxidative damage to sperm. The aim of this study was to evaluate the effect of ultraviolet (UV) irradiation on boar sperm oxidative damage and sperm quality. Boar semen was held at 17°C for 0 hour and 3 hours, respectively, and irradiated with different intensities of ultraviolet (0, 0.2, 0.4, 0.6, 0.8, 1.0 J/cm²). Sperm quality was determined by assessing the sperm motility, viability, mitochondrial membrane potential (MMP), acrosome integrity and reactive oxygen species (ROS) level. The results showed that only ROS level of UV irradiated sperm held at 17°C for 3 hours was significantly lower than that of 0 hour holding time. Increasing the intensity of UV irradiation significantly reduced the total motility, progress motility, mitochondrial membrane potential and viability of sperm, and increased the ROS level. The UV intensity of 0.6 J/cm² irradiation could reduce 50% sperm total motility, but it had no effect on the sperm acrosome integrity. In the conclusion, UV irradiation caused oxidative damage to sperm and reduced sperm quality. There's dose-effect relation within UV irradiation. However, boar semen held at 17°C for 3 hours could effectively reduce the oxidative damage to sperm.
- Subjects
ULTRAVIOLET radiation; BOARS; FROZEN semen; SPERM motility; ACROSOMES; OXIDATIVE stress
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p14
- ISSN
0253-9187
- Publication type
Article