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- Title
Comparison of different concentrations of chlorhexidine-iodophor composite solution on human skin fibroblasts.
- Authors
YiXin, Luo; ShaoNa, Niu; LiFang, Mai; XingZhou, Liu; Chuan, Yang
- Abstract
Objective: Chlorhexidine-iodophor (CHX-IP) composite solution is a polymer of chlorhexidine and iodophor produced with new technology, for use in diabetic foot infection. However, the effect of CHX-IP on the growth activity of fibroblasts remains unknown, thus the effects of different concentrations of CHX-IP composite solution on the viability and micromorphology of human skin fibroblasts were studied in vitro cell culture in this study. Method: A cell viability assay was applied to calculate cell viability and an inverted fluorescence microscope was used to observe cell morphology over five days. Results: The results showed that the toxic effect of CHX-IP on fibroblasts was solution concentration-dependent and decreased over time. When the concentration of CHX-IP was 5.0mg/ml, 2.5mg/ml, 0.625mg/ml, 0.15625mg/ml, 0.078125mg/ml or 0mg/ml, the difference of optical density (OD) value on different days was statistically significant (p<0.05). There were statistically significant differences in the OD value of fibroblasts among different concentrations of CHX-IP on: day 2 (F=4.809, p=0.004); day 3 (F=21.508, p<0.001); day 4 (F=63.952, p<0.001); and day 5 (F=160.407, p<0.001). In addition, a concentration of 5.0mg/ml CHX-IP resulted in a fibroblastic viability rate of 0% on day 4, when CHX-IP was diluted to 2.5mg/ml or 1.25 mg/ml, fibroblastic viability rate decreased to 0% day 5. However, when the CHX-IP was diluted to 0.15625mg/ml or 0.078125mg/ml, the fibroblastic cell viability rate increased slightly on day 5. The morphology of cells observed under microscope indirectly supported this result. Conclusion: The findings of this study showed that the toxic effect of CHX-IP on fibroblasts was solution concentration-dependent and decreased over time.
- Subjects
DRUG efficacy; IN vitro studies; FIBROBLAST growth factors; FIBROBLASTS; COMBINATION drug therapy; CELL culture; SKIN; MICROSCOPY; DRUG overdose; ONE-way analysis of variance; IODOPHORS; CELL survival; COMPARATIVE studies; MOLECULAR biology; CELLS; TOXICITY testing; DESCRIPTIVE statistics; CHLORHEXIDINE; STERILIZATION (Disinfection); DATA analysis software; DOSAGE forms of drugs; PHARMACODYNAMICS; EVALUATION
- Publication
Journal of Wound Care, 2024, Vol 33, Issue Sup2a, pxxxii
- ISSN
0969-0700
- Publication type
Article
- DOI
10.12968/jowc.2024.33.Sup2a.xxxii