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- Title
Morphological and Functional Changes in the Peritumoral Adipose Tissue of Colorectal Cancer Patients.
- Authors
Zoico, Elena; Rizzatti, Vanni; Darra, Elena; Budui, Simona Luciana; Franceschetti, Guido; Vinante, Fabrizio; Pedrazzani, Corrado; Guglielmi, Alfredo; De Manzoni, Giovanni; Mazzali, Gloria; Rossi, Andrea Petronio; Fantin, Francesco; Zamboni, Mauro
- Abstract
<bold>Objective: </bold>The role of peritumoral adipose tissue (AT) has not been extensively studied in colorectal cancer (CRC).<bold>Methods: </bold>This study was conducted in 20 male subjects undergoing elective surgery for CRC. The differences between the peritumoral visceral adipose tissue (P-VAT), visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) of the patients were described via immunohistochemistry and molecular biology analyses. The interactions between adipocytes and a colon cancer cell line were also investigated by using an in vitro coculture system.<bold>Results: </bold>The analyses revealed that adipocytes near the tumor were significantly smaller than the adipocytes from other sites. The P-VAT was preferentially infiltrated by a CD68+/CD163+/IDO- macrophage subset with a prevalent reparative inflammatory response, while the macrophages identified in VAT and SAT mainly presented inflammatory features. Furthermore, the P-VAT presented a higher expression of adiponectin compared with other sites. Morphological analysis in vitro showed that after a few days of coculture, 3T3-L1 adipocytes were reduced in number and size with an increase in lipolysis rate and dedifferentiation phenomena.<bold>Conclusions: </bold>This study reveals important morphological and functional changes in the AT surrounding the tumor as an increase in lipolysis and in adiponectin-producing adipocytes, preferentially infiltrated by a macrophage subset, with prevalent reparative inflammatory response.
- Subjects
ADIPOSE tissues; IMMUNOHISTOCHEMISTRY; GENE expression; INFLAMMATION; NEOVASCULARIZATION; COLON tumors; RECTUM tumors
- Publication
Obesity (19307381), 2017, Vol 25, pS87
- ISSN
1930-7381
- Publication type
journal article
- DOI
10.1002/oby.22008