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- Title
Omentin treatment of epicardial fat improves its anti-inflammatory activity and paracrine benefit on smooth muscle cells.
- Authors
Fernández‐Trasancos, Ángel; Agra, Rosa María; García‐Acuña, Jose María; Fernández, Ángel Luis; González‐Juanatey, José Ramón; Eiras, Sonia
- Abstract
<bold>Objective: </bold>Epicardial adipose tissue (EAT) in coronary artery disease is insulin resistant and has a proinflammatory profile. This study examined the regulation of EAT by exogenous omentin and its consequence on vascular cells.<bold>Methods: </bold>Stromal vascular cells (SC) of EAT and subcutaneous adipose tissue (SAT) from patients who underwent heart surgery were cultured and exposed to adipogenic factors with or without omentin. Proinflammatory cytokine regulation by omentin was analyzed in SC and mature adipocytes. Glucose uptake by EAT and SAT explants was determined after insulin, omentin, or combined treatment. Human vascular cells were exposed to secretomes of SC, with and without omentin treatment. Migration of smooth muscle cells and expression of adhesion molecules were determined by wound healing or real-time polymerase chain reaction, respectively.<bold>Results: </bold>Omentin treatment raised adipogenesis-induced adiponectin levels on SC of EAT and reduced TNF-α expression levels (0.58 ± 0.14-fold change; P = 0.034) in mature adipocytes. Omentin improved the insulin activity of EAT and SAT explants from cardiovascular disease patients. Finally, secretomes of SC under omentin treatment reduced the migration of smooth muscle cells.<bold>Conclusions: </bold>Exogenous omentin might support a cardioprotective role through its effect on EAT regarding glucose uptake, anti-inflammatory response, and its paracrine role on smooth muscle cells.
- Subjects
ANTI-inflammatory agents; INSULIN; ADIPONECTIN; FAT cells; PARACRINE mechanisms; MUSCLE cells; BARIATRIC surgery; OBESITY treatment; ADIPOSE tissues; CARDIOVASCULAR diseases; CELL physiology; CELLS; GENE expression; PHARMACODYNAMICS
- Publication
Obesity (19307381), 2017, Vol 25, Issue 6, p1042
- ISSN
1930-7381
- Publication type
journal article
- DOI
10.1002/oby.21832