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- Title
白菜型油菜IPT 基因家族鉴定及表达分析.
- Authors
刘博; 王旺田; 马骊; 武军艳; 蒲媛媛; 刘丽君; 方彦; 孙万仓; 张岩; 刘睿敏; 曾秀存
- Abstract
Isopentenyltransferase (IPT) is the first limiting enzyme for cytokinin biosynthesis, so to analyze the function of the IPT gene family in Brassica rapa L., a total of 13 BrIPT genes were identified from the genome of Brassica rapa L. by bioinformatics, which were heterogeneously distributed on 7 chromosomes. These BrIPT genes were divided into 4 substrates and each BrIPT gene included 8~10 conserved motifs and 1~2 UTR regions. The promoter regions of BrIPT genes contained numerous responsive elements. The BrIPT genes were regulated by environmental factors, biohormones, defense and stress. The qRT-PCR results showed that the tRNA-IPT genes BrIPT4, BrIPT6 and BrIPT9 were expressed in various parts of Brassica rapa L.. The expressions of IPT genes in Brassica rapa L. were higher at the mature stage than those at the seedling stage. The above results provided some basis for subsequent in-depth studies on the physiological and biochemical functions of IPT gene family members.
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2024, Vol 26, Issue 2, p56
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2022.0373