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- Title
E-selectin gene in essential hypertension: a case-control study.
- Authors
Srivastava, Kamna; Chandra, Sudhir; Narang, Rajiv; Bhatia, Jagriti; Saluja, Daman
- Abstract
Background Hypertension is associated with endothelial cell dysfunction. E-selectin, an endothelial cell adhesion molecule, is specific for endothelial cell activation. Polymorphism in E-selectin gene has recently been identified among which Leu554Phe E-selectin gene polymorphism is least investigated in essential hypertension. This study reports the association of E-selectin gene Leu554Phe polymorphism and the expression of E-selectin gene in patients with essential hypertension. Materials and methods We analysed the Leu554Phe polymorphism and expression of E-selectin gene in 250 patients with essential hypertension and 250 normal healthy controls. Genotyping of Leu554Phe polymorphism was performed by polymerase chain reaction-restriction fragment length polymorphism ( PCR- RFLP), and the expression of E-selectin gene at mRNA and protein levels were carried out by real-time PCR and Western blot, respectively. Results A significant association of E-selectin genotypes ( CT + TT) with essential hypertension ( P < .0001, Odds ratio = 2.2 [1.58-3.24] at 95% CI) was observed. The expression of mRNA for E-selectin gene in patients with essential hypertension was ~12-fold higher as compared to control. We observed an elevated level of E-selectin protein expression (up to 1.9 times) in patients as compared to controls. Conclusions A significant association of E-selectin (Leu554Phe) gene and increased expression of E-selectin gene at mRNA and protein levels in patients might be related to the genetic predisposition to develop essential hypertension.
- Subjects
SELECTIN genetics; HYPERTENSION genetics; ENDOTHELIAL cells; SINGLE nucleotide polymorphisms; GENE expression; CELL adhesion molecule genetics
- Publication
European Journal of Clinical Investigation, 2018, Vol 48, Issue 1, pn/a
- ISSN
0014-2972
- Publication type
Article
- DOI
10.1111/eci.12868