We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Expression of Interferon-tau mRNA in Bovine Embryos Derived from Different Procedures.
- Authors
Yao, N.; Wan, P-C.; Hao, Z-D.; Gao, F-F.; Yang, L.; Cui, M-S.; Wu, Y.; Liu, J-H.; Liu, S.; Chen, H.; Zeng, S-M.
- Abstract
Contents Interferon-tau (IFN-τ) is a secreted conceptus protein which plays a critical role in the establishment of ruminant pregnancy by its antiluteolytic and antiviral effects. In the present study, we hypothesized that IFN-τ expression was temporally and spatially regulated in different pre-implantation embryos and the levels of IFN-τ expression were different among bovine embryos derived from parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). By using in situ hybridization with Digoxingenin (DIG)-labelled IFN-τ cDNA as a probe, we detected IFN-τ mRNA in bovine embryos from days 3 to 9 in culture. However, the timing of the initiation of IFN-τ mRNA expression was different among PA, IVF and SCNT embryos. Interferon-τ mRNA was first expressed in 16-cell stage IVF embryos on day 4, in SCNT morula on day 5 and early PA blastocyst on day 6. Semi-quantitative RT-PCR analysis showed that the expression levels of IFN-τ mRNA did not differ significantly among IVF, SCNT and PA embryos on day 7. In addition, freezing and thawing did not have a major impact either on IFN-τ mRNA expression in IVF or in vivo-produced bovine blastocysts.
- Subjects
INTERFERONS; EMBRYOS; PROTEINS; RUMINANTS; PREGNANCY
- Publication
Reproduction in Domestic Animals, 2009, Vol 44, Issue 1, p132
- ISSN
0936-6768
- Publication type
Article
- DOI
10.1111/j.1439-0531.2007.01009.x