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- Title
Class II β-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells.
- Authors
Lee, Ju‐Han; Kong, Su‐Kang; Wu, Zheng‐Sheng; Wu, Qiang; Choe, Jongseon; Cho, Hyun Yee; Ha, Seung Yeon; Kee, Sun Ho; Kim, Young‐Sik
- Abstract
J Oral Pathol Med (2010) 39: 533–539 Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β-tubulin were carried out. Results: Class II β-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.
- Subjects
TUBULINS; DENDRITIC cells; IMMUNOHISTOCHEMISTRY; LYMPHOID tissue; LYMPHOMAS
- Publication
Journal of Oral Pathology & Medicine, 2010, Vol 39, Issue 7, p533
- ISSN
0904-2512
- Publication type
Article
- DOI
10.1111/j.1600-0714.2009.00865.x