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- Title
Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus.
- Authors
Karagouni, E. E.; Dotsika, E. N.; Sklavounou, A.
- Abstract
Different activation parameters of peripheral blood mononuclcar cells (PBMC) from 31 patients with oral lichen planus (OLP) were examined and compared with 23 healthy donors. Impaired spontaneous (450 ± 241 vs 1290 ± 480 cpm) and mitogen-induced (39580 ± 14470 vs 67000 ± 11810 cpm) lymphocyte blastogenesis was observed in OLP patients. Furthermore, reduced cytokine production was found after phytohemagglutinin A (PHA) stimulation for all cytokines studiedturnout necrosis factor α (TNFα, 432.2 ± 73.4 vs 979.8 ± 46.3 units/ml), interleukin 2 (IL-2, 156.2 ± 14.9 vs 572.6 &pusmn; 12.9 pg/ml), interferon 7 (IFNγ, 48.54 ± 11.9 vs 82.6 ± 12.4 pg/ml) and interleukin 6 (IL-6, 253.6 ± 57.7 vs 1.419.0 ± 279.6 units/ ml)-except for interleukin I β (IL-Iβ) and lymphotoxin (LT). In contrast, unstimulated culture supernatants showed increased TNFa (38.2 ± 13.1 vs 8.1 ± 0.2 units, ml). LT (10.2 ± 2.2 units/mi vs. < 0.4)and IL-6 (18.5 ± 5.6 units/mi vs < 0.5) activity. Similarly. elevated concentrations of TNF α (19.6 ± 6.3 units/mi) and IL-6 122.9 ± 4.7 units/mi) were deteclcd in the sera of OLP patients. Combination of PHA and phorbol myristate acetate (PMA) could restore OLP proliferative T cell response and cytokine production to the level of healthy donors, whereas exogenous recombinant human IL-2 (rhul L-2) plus PMA did not seem to be an effective stimulant for OLP T cells. These results indicate an alteration in the immune condition of OLP patients and an impairment in T lymphocyte function.
- Subjects
BLASTOGENESIS (Embryology); CYTOKINES; INTERLEUKINS; IMMUNOREGULATION; LICHEN planus; EMBRYOLOGY
- Publication
Journal of Oral Pathology & Medicine, 1994, Vol 23, Issue 1, p28
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1994.tb00250.x