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- Title
High-yield production of 1,3-propanediol from glycerol by metabolically engineered <italic>Klebsiella pneumoniae</italic>.
- Authors
Lee, Jung Hun; Oh, Min-Kyu; Jung, Moo-Young
- Abstract
Background: Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield. Results: <italic>Klebsiella pneumoniae</italic> was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in <italic>K</italic>. <italic>pneumoniae</italic>. Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the <italic>dha</italic> operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol. Conclusions: <italic>Klebsiella pneumoniae</italic> is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies.
- Subjects
GLYCERIN; BIODIESEL fuels; KLEBSIELLA pneumoniae; MANNITOL; PROPYLENE glycols
- Publication
Biotechnology for Biofuels, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1754-6834
- Publication type
Article
- DOI
10.1186/s13068-018-1100-5