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- Title
Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitum alkaloids in Shen- Fu decoction.
- Authors
Na Guo; Mingtao Liu; Dawei Yang; Ying Huang; Xiaohong Niu; Ruifan Wu; Ying Liu; Guizhi Ma; Deqiang Dou
- Abstract
Background Shen-Fu decoction is a traditional Chinese medicine prescription with a 3:2 ratio of Radix Ginseng and Fuzi (Radix Aconiti lateralis praeparata). Ginsenosides and alkaloids are considered to be the main active components of Shen-Fu decoction. However, no analytical methods have been used to quantitatively analyse both components in Shen-Fu decoction simultaneously. Results We successfully developed a rapid resolution liquid chromatography coupled with tandem mass spectrometry (RRLC-MS/MS) method for the simultaneous analysis of seven ginsenosides and three aconitum alkaloids in Shen-Fu decoction, the decoction of Radix ginseng and Fuzi (Radix Aconiti lateralis praeparata). Chromatogrpahic separation by RPLC was achieved using a reversed-phase column and a water/acetonitrile mobile phase, containing 0.05% formic acid and using a gradient system. The method was optimized to allow for simultaneous analysis of all analytes in 11minutes without the need for baseline resolution of the components. Furthermore, the separation demonstrated good linearity (r > 0.9882), repeatability (RSD < 7.01%), intra- and inter-day precisions (RSD < 5.06%) and high yields of recovery (91.13-111.97%) for ten major constituents, namely ginsenoside-Re, Rg1, Rb1, Rc, Rb2, Rd, Rf, aconitine, hypacoitine and mesaconitine. Conclusions The developed method could be used as a rapid and reliable approach for assessment of the quantity of the major constituents in Shen-Fu decoction.
- Subjects
GINSENOSIDES; MONKSHOODS; THERAPEUTIC use of alkaloids; CHINESE medicine; LIQUID chromatography-mass spectrometry
- Publication
Chemistry Central Journal, 2013, Vol 7, Issue 1, p1
- ISSN
1752-153X
- Publication type
Article
- DOI
10.1186/1752-153X-7-165