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- Title
Gonadotropin-Releasing Hormone Receptor Expression in Human Spinal Cord.
- Authors
Díaz-Galindo, Carmen; Calderón-Vallejo, Denisse; Hernández-Jasso, Irma; Cervantes-García, Daniel; Martínez-Díaz, Diego; Ibarra-Martínez, David; Muñoz-Ortega, Martín; Quintanar, J. Luis
- Abstract
The expression of the gonadotrophin-releasing hormone receptor expression on pituitary gonadotrophs in humans is well characterized. In nervous system they have also been found in hippocampi and cerebral cortex. However, gonadotrophin-releasing hormone receptor expression in human spinal cord has not been reported. This study was to analyze the gonadotrophin-releasing hormone receptor expression in human spinal cord by immunohistochemistry, mRNAs by reverse transcriptase polymerase chain reaction, cDNA cloning and Western blot. The results show immunoreactive material to gonadotrophin-releasing hormone receptor in motoneurons of the spinal cord. Further, the study revealed that spinal cord expressed the gonadotrophin-releasing hormone receptor mRNA. The amplicon sequence corresponds to 100% of identity to GenBank. In Western blot, a band of 37 kDa were found in extracts of spinal cord and placenta as a control. In conclusion, human spinal cord expresses gonadotrophin-releasing hormone receptor analyzed through immunohistochemistry, the expression of its mRNA, cloning its cDNA and Western blot analysis. The presence of gonadotrophin-releasing hormone receptor in the spinal cord suggests the possibility of an extrapituitary functional role independent of reproductive system.
- Subjects
LUTEINIZING hormone releasing hormone receptors; SPINAL cord; REVERSE transcriptase polymerase chain reaction; HORMONE receptors; PREOPTIC area; WESTERN immunoblotting
- Publication
Neurochemical Research, 2021, Vol 46, Issue 2, p165
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-020-03178-w