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- Title
Nuclear localization of propiece IL-1α in HeLa cells.
- Authors
Yoshihiro Kudo; Takaaki Tamagawa; Kensuke Nishio; Tadayoshi Kaneko; Yoshiyuki Yonehara; Mariko Tsunoda; Kudo, Yoshihiro; Tamagawa, Takaaki; Nishio, Kensuke; Kaneko, Tadayoshi; Yonehara, Yoshiyuki; Tsunoda, Mariko
- Abstract
<bold>Purpose: </bold>The study aimed to examine the nuclear localization of propiece interleukin (IL)-1α (ppIL-1α) and extracellular release rates of ppIL-1α, pIL-1α, and mIL-1α.<bold>Methods: </bold>The subcellular localization of IL-1α molecules was observed in HeLa cells transfected with green fluorescent protein (GFP)-tagged IL-1α. Extracellular release efficiency was examined using N-terminal HiBiT-tagged IL-1α. The nuclear localization status of ppIL-1α was examined by incubating ppIL-1α transfectants with 0.1% Triton X-100 solution or with complete medium on ice.<bold>Results: </bold>The results indicated the diffuse cytoplasmic and nuclear localization for m and p and ppIL-1, respectively. All IL-1α forms were released from the cells even in the steady state, and the release efficiency was 25%, 13%, and 8% for mIL-1α, pIL-1α, and ppIL-1α, respectively. Under oxidative stress condition, GFP-mIL-1α was totally diminished, but weak staining of GFP-pIL-1α and GFP-ppIL-1α was detected; nuclear localization of GFP-ppIL-1α was completely abolished by 0.1% Triton X-100 treatment, however, it remained in the nucleus after culture in complete medium on ice.<bold>Conclusion: </bold>The results of this study showed that ppIL-1α was localized in the nucleus and released extracellularly even in the steady state. Moreover, its cellular localization is not firm, and it is presumed to be floating in the nucleoplasm.
- Subjects
CELL nuclei; PSYCHOLOGICAL tests; CELLS; FAMILY relations
- Publication
Journal of Oral Science, 2022, Vol 64, Issue 2, p151
- ISSN
1343-4934
- Publication type
journal article
- DOI
10.2334/josnusd.21-0540