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- Title
Release of Cytokines and Soluble Cell Surface Molecules by PBMC after Activation with the Bispecific Antibody CDxCD19.
- Authors
Klein, S. C.; Boer, L. H.; De Weger, R. A.; De Gast, G. C.; Bast, E. J. E. G.
- Abstract
Bispecific antibodies (BsAb) consist of two different heavy and light chains and may bind to two different antigens present on different cell types. With their dual specificity BsAb may recognize effector cells (e.g. T cells) on one hand and tumour cells (e.g. malignant B cells) on the other hand. The authors analysed whether T cell activation and subsequent killing of malignant B cells mediated by the bispecific antibody CD3xCD19 was reflected by the release of cytokines. In addition, the authors investigated whether the <em>in vitro</em> cytokine release was similar to that observed <em>in vivo</em> in the patients treated with BsAb. The <em>in vitro</em> release of cytokines into the supernatant of cell cultures of peripheral blood mononuclear cells (PBMC) and malignant B cells was measured after incubation with either the bispecific antibody CD3 x CD19 or the monospecific anti- CD3 (aCD3) antibody in the presence or absence of interleukin (IL)-2. Release of tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-6, IL-8, IL-10, soluble (s) CD4, sCD8 and sCD25 by PBMC was equal under both conditions and could be used as an indicator for T cell activation. However, the cytokine pattern and level did not correlate with the cytotoxic capacity, which was 4 logs higher with BsAb + IL-2 compared to aCD3 + IL-2. The <em>in vitro</em> pattern of cytokine release was similar to that observed <em>in vivo</em> in the serum of patients treated with BsAb and IL-2, indicating the possibility of predicting cytokine release in future patients with other therapeutic regimens.
- Subjects
BISPECIFIC antibodies; ANTIGENS; T cells; B cells; CYTOKINES
- Publication
Scandinavian Journal of Immunology, 1997, Vol 46, Issue 5, p452
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.1997.d01-151.x