We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Diaminobenzidine Cytochemistry in Unfixed Human Epidermis: A Marker for Epidermal Differentiation and for Mitochondria.
- Authors
Dubertret, Louis; Breton-Gorius, Janine; Fosse, Michele; Touraine, René
- Abstract
Incubation of unfixed and unfrozen slices of skin in diaminobenzidine allows visualization of a peroxidatic activity in perinuclear envelope of suprabasal keratinocytes undergoing orthokeratotic differentiation. Basal keratinocytes and melanocytes are always negative. This enzyme is absent in mucous and parakeratotic (psoriatic) differentiation. Mitochondria are also strongly stained by this technique and it was shown that the number of epidermal mitochondria is greatly increased in psoriatic lesions.
- Subjects
SKIN; CYTOCHEMISTRY; KERATINOCYTES; EPIDERMIS; MITOCHONDRIA; PSORIASIS
- Publication
Journal of Investigative Dermatology, 1982, Vol 78, Issue 6, p477
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12510235