We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Induction of effective antitumor immune responses in a mouse bladder tumor model by using DNA of an α antigen from mycobacteria.
- Authors
Kuromatsu, Isao; Matsuo, Kazuhiro; Takamura, Shiki; Kim, Gisen; Takebe, Yutaka; Kawamura, Juichi; Yasutomi, Yasuhiro
- Abstract
One of the main objectives of cancer immunotherapy is the activation and increase in number of antitumor effector cells. Recently, genetically modified tumor cell vaccines have been proposed for elicitation of antitumor effector cells. Native α antigen (α Ag) (also known as MPT59 and antigen 85B) of mycobacteria, which cross-reacts among mycobacteria species, may play an important biological role in host-pathogen interaction because it elicits various helper T-cell type 1 immune responses. To assess the induction of antitumor immune responses by α Ag, mouse tumor cell lines transfected with cDNA of α Ag from Mycobacterium kansasii were established, and the possibility of producing a tumor cell vaccine for induction of antitumor effects was explored. Transfection of tumor cell lines with an α Ag gene lead to primary tumor rejection and the establishment of protective immunity to nontransfected original tumor cell lines in Mycobacterium bovis bacillus Calmette-Guérin (BCG)-primed and unprimed mice. Mice immunized with tumor cell lines transfected with the α Ag gene showed delayed-type hypersensitivity responses in vivo and proliferative responses together with induction of interferon-γ of spleen cells against nontransfected wild-type tumor cell lines in in vitro experiments. Moreover, immunization of mice with α Ag-expressing tumor cells elicited tumor-specific and cytotoxic T lymphocyte (CTL) epitope peptide-specific CD8[sup +] CTLs. The results of this study provided evidence of the potential usefulness of α Ag in tumor cell vaccines.
- Subjects
CANCER immunotherapy; ANTIGENS; MYCOBACTERIA
- Publication
Cancer Gene Therapy, 2001, Vol 8, Issue 7, p483
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/sj.cgt.7700330