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- Title
Validation of high-throughput genotoxicity assay screening using γH2AX in-cell western assay on HepG2 cells.
- Authors
Khoury, Laure; Zalko, Daniel; Audebert, Marc
- Abstract
In vitro genotoxicity tests used in regulatory toxicology studies are sensitive, but the occurrence of irrelevant positive results is high compared with carcinogenicity studies in rodents. Current in vitro genotoxicity tests are also often limited by relatively low throughput. The aim of this study was to validate an in vitro genotoxic assay in a 96-well plate format that allows the simultaneous examination of cytotoxicity and genotoxicity. The test is based on the quantification of the phosphorylation of the histone H2AX (γH2AX), which reflects a global genotoxic insult, using the In-Cell Western technique. The assay was evaluated on HepG2 cells by testing a list of 61 compounds recommended by the European Center for the Validation of Alternative Methods (ECVAM), whose genotoxic potential has already been characterized. The γH2AX assay on HepG2 cell line was highly sensitive: 75% of the genotoxic compounds gave a positive result, and specific: 90-100% of nongenotoxic compounds gave negative results. Compared with the micronucleus genotoxicity assay using the same cell line and test compounds, the γH2AX assay was more sensitive and specific. In sum, the high-throughput γH2AX assay described here can accurately detect simultaneously the genotoxic and the cytotoxic potential of compounds with different modes of mutagenic action, notably those who required metabolic activation. The use of this assay in the early discovery phase of drug development may prove to be a valuable way to assess the genotoxic potential of xenobiotics. Environ. Mol. Mutagen. 54:737-746, 2013. © 2013 Wiley Periodicals, Inc.
- Subjects
GENETIC toxicology; CARCINOGENICITY; LABORATORY rodents; CELL lines; DRUG development
- Publication
Environmental & Molecular Mutagenesis, 2013, Vol 54, Issue 9, p737
- ISSN
0893-6692
- Publication type
Article
- DOI
10.1002/em.21817