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- Title
Chiorophyllin Significantly Reduces Benzo [a] pyrene - DNA Adduct Formation and Alters Cytochrome P450 1A1 and 1B1 expression and EROD Activity in Normal Human Mammary Epithelial Cells.
- Authors
Keshava, Channa; Divi, Rao L.; Einem, Tracey L.; Richardson, Diana L.; Leonard, Sarah L.; Keshava, Nagalakshmi; Poirier, Miriam C.; Weston, Ainsley
- Abstract
The article presents a hypothesis which states that chlorophyllin (CHLN) can reduce benzo[a]pyrene-DNA (BP-DNA) adduct levels. It involves the measurement of BP-DNA adducts through the use of chemiluminescence immunoassay. In an aim to analyze metabolic mechanisms, there was a monitoring of expression using Affymetrix microarray. The hypothesis shows not only BP-induced up-regulation of CYP1A1 and CYP1B1 expression, but the upregulation of groups of interferon-inducible, inflammation and signal transduction genes as well. It shows that there was a decline in the expression of many genes when it comes to incubation of cells with CHLN and BP in any combination.
- Subjects
CHLOROPHYLLIN; MUTAGENESIS; BENZOPYRENE; DNA; GENE expression; CHEMILUMINESCENCE immunoassay; GENETIC regulation; POLYCYCLIC aromatic hydrocarbons; HYPOTHESIS
- Publication
Environmental & Molecular Mutagenesis, 2009, Vol 50, Issue 2, p134
- ISSN
0893-6692
- Publication type
Article
- DOI
10.1002/em.20449