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- Title
Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro.
- Authors
Wu, Xian-Lin; Ju, Da-Hong; Chen, Jia; Yu, Bin; Liu, Kang-Li; He, Jin-Xiong; Dai, Cong-Qi; Wu, Sha; Chang, Zhou; Wang, You-Ping; Chen, Xiao-Yin
- Abstract
Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway. Graphical Abstract: [Figure not available: see fulltext.]
- Subjects
H1N1 influenza; CHINESE medicine; DENDRITIC cells; EPITHELIAL cells; CYTOKINES; MESSENGER RNA
- Publication
Current Microbiology, 2013, Vol 67, Issue 4, p431
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-013-0381-y