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- Title
Combating against methicillin-resistant S taphylococcus aureus - two fatty acids from Purslane ( P ortulaca oleracea L.) exhibit synergistic effects with erythromycin.
- Authors
Chan, Ben C.L.; Han, X.Q.; Lui, Sau Lai; Wong, C.W.; Wang, Tina B.Y.; Cheung, David W.S.; Cheng, Sau Wan; Ip, Margaret; Han, Simon Q.B.; Yang, Xiao‐Sheng; Jolivalt, Claude; Lau, Clara B.S.; Leung, Ping Chung; Fung, Kwok Pui
- Abstract
Objectives The aims of this study were to identify the active ingredients from P ortulaca oleracea L. ( PO) that could provide synergism with antibiotics against methicillin-resistant S taphylococcus aureus ( MRSA) and their possible mechanisms of resistance inhibition. Methods High-speed counter-current chromatography ( HSCCC) coupled with gas chromatography-mass spectrometry and a panel of laboratory MRSA strains were used for checkerboard and efflux inhibitory assays. Key findings Linoleic and oleic acids were identified from HSCCC fraction 18 of PO with synergistic antibacterial activity when combined with erythromycin against RN4220/ pUL5054. Ethidium bromide efflux inhibitory studies revealed that linoleic and oleic acids may interfere the activity of MsrA pump. By comparing among a panel of linoleic and oleic acids analogues, unsaturated fatty acids in salt form with cis configuration and an increase in number of double bonds were found to further increase the antibacterial activity when used alone or in combination with antibiotics. Conclusion This study reported for the first time that two active ingredients, namely linoleic and oleic acids, were identified from PO with synergistic antibacterial activity when combined with erythromycin against MRSA RN4220/ pUL5054 and possibly act by inhibiting the efflux pumps of the bacteria cells.
- Subjects
METHICILLIN-resistant staphylococcus aureus; PORTULACA oleracea; DRUG synergism; FATTY acid synthesis; ERYTHROMYCIN; OLEIC acid; METHICILLIN resistance
- Publication
Journal of Pharmacy & Pharmacology, 2015, Vol 67, Issue 1, p107
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12315