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- Title
Critical appraisal of E test for the detection of fluoroquinolone resistance.
- Authors
Jones, Roland N.; Erwin, Meridith E.; Croco, Janet L.; Jones, R N; Erwin, M E; Croco, J L
- Abstract
The ability of E test to accurately detect fluoroquinolone resistance was compared with an agar dilution reference method. Nearly 300 isolates belonging to 26 different species (62.5% with documented ciprofloxacin resistance) were tested with ciprofloxacin, fleroxacin, levofloxacin, norfloxacin, ofloxacin, and sparfloxacin. In contrast to earlier reports, E test MIC values for pneumococci and all rapid growing aerobes were routinely higher (approximately 0.5 log2 dilution step) than agar dilution results. The E test stable-gradient method also efficiently indentified fluoroquinolone-resistant subpopulations which were not detected by the reference procedure with the standard inoculum. Categorical agreement for 1710 test comparisons was approximately 90% with no very major, false-susceptible errors. We found the E test to be a valid, reproducible method for fluoroquinolone susceptibility testing that provided quantitative results and produced a conservative (1.6% false-resistant results) assessment of susceptibility of bacteria including isolates of Streptococcus pneumoniae and Pseudomonas aeruginosa to compounds in this antimicrobial class.
- Subjects
ANTI-infective agents; BACTERIAL growth; DRUG resistance in microorganisms; MICROBIAL sensitivity tests; MICROBIOLOGICAL techniques; QUINOLONE antibacterial agents; RESEARCH evaluation; STATISTICS; DATA analysis; PHARMACODYNAMICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1996, Vol 38, Issue 1, p21
- ISSN
0305-7453
- Publication type
journal article
- DOI
10.1093/jac/38.1.21