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- Title
Endosomal signalling via exosome surface TGFβ-1.
- Authors
Shelke, Ganesh Vilas; Yin, Yanan; Jang, Su Chul; Lässer, Cecilia; Wennmalm, Stefan; Hoffmann, Hans Jürgen; Li, Li; Gho, Yong Song; Nilsson, Jonas Andreas; Lötvall, Jan
- Abstract
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.
- Subjects
TRANSFORMING growth factors; HUMAN stem cells; MESENCHYMAL stem cells; EXOSOMES; MAST cells; ENDOSOMES
- Publication
Journal of Extracellular Vesicles, 2019, Vol 8, Issue 1, p1
- ISSN
2001-3078
- Publication type
Article
- DOI
10.1080/20013078.2019.1650458