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- Title
Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk.
- Authors
García, Juan Ignacio; Kelley, Holden V.; Meléndez, Johanna; de León, Rosa Alejandra Alvarez; Castillo, Alejandra; Sidiki, Sabeen; Yusoof, Kizil A.; Nunes, Elizabete; Téllez, Cesar López; Mejía-Villatoro, Carlos Rodolfo; Ikeda, Janet; García-Basteiro, Alberto L.; Wang, Shu-Hua; Torrelles, Jordi B.
- Abstract
Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with α-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.
- Subjects
LIPOARABINOMANNANS; TUBERCULOSIS diagnosis; IMMUNODIAGNOSIS; MILK analysis; MANNOSIDASES
- Publication
Scientific Reports, 2019, Vol 9, Issue 1, pN.PAG
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-019-54537-9