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- Title
Molecular Characterization of a Novel Integrative Conjugative Element ICE Hpa1 in Haemophilus parasuis.
- Authors
Sun, Hua-Run; Cui, Xiao-Die; Liu, Xiao-Kang; Li, Shuai-Hua; Yi, Kai-Fang; Pan, Yu-Shan; Wu, Hua; Yuan, Li; Hu, Gong-Zheng; He, Dan-Dan
- Abstract
ICE Hpa1 was identified in the genome of a serovar 8 Haemophilus parasuis ST288 isolate YHP170504 from a case of swine lower respiratory tract infection. The aim of the present study was to characterize the integrative conjugative element ICE Hpa1 and its multiresistance region. Susceptibility testing was determined by broth microdilution and the complete ICE Hpa1 was identified by WGS analysis. The full sequence of ICE Hpa1 was analyzed with bioinformatic tools. The presence of ICE Hpa1 , its circular intermediate and integration site were confirmed by PCR and sequence analysis. Transfer of ICE Hpa1 was confirmed by conjugation. ICE Hpa1 has a size of 68,922 bp with 37.42% GC content and harbors 81 genes responsible for replication and stabilization, transfer, integration, and accessory functions, as well as seven different resistance genes [ bla Rob–3, tet (B), aphA1 , strA , strB , aac(6)′-Ie-aph(2′)-Ia , and sul2 ]. Conjugation experiments showed that ICE Hpa1 could be transferred to H. parasuis V43 with frequencies of 6.1 × 10–6. This is the first time a multidrug-resistance ICE has been reported in H. parasuis. Seven different resistance genes were located on a novel integrative conjugative element ICE Hpa1 , which suggests that the ICE Hpa1 is capable of acquiring foreign genes and serving as a carrier for various resistance genes.
- Subjects
HAEMOPHILUS; RESPIRATORY infections; ICE; HAEMOPHILUS diseases; RESPIRATORY syncytial virus
- Publication
Frontiers in Microbiology, 2020, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.01884