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- Title
Effects of Volatile Oils of Sichuan Citrisarcodactylis fructus on Proliferation and Apoptosis of MCF-7 Human Breast Cancer Cells.
- Authors
Siwei CHEN; Dan ZHANG; Zhuo ZHANG; Xin YU; Fang WANG
- Abstract
[Objectives] To study the effects of volatile oils of Sichuan Citrisarcodactylis fructus on proliferation and apoptosis of MCF-7 human breast cancer cells. [Methods] CCK-8 method was used to measure the cell proliferation, the flow cytometry was used to measure changes in ceH cycle, Western blot was used to detect p53 and cyclin D1 activity changes, TUNEL method was used to measure percentage of apoptotic ceHs, inverted phase contrast microscope and transmission electron microscope were used to observe morphology of MCF-7 ceHs treated with different concentrations. [Results] When the concentration was 5 - 50 μ.mol/L, the ceH proliferation inhibition rate increased significantly (P <0.05), G2/M phase decreased significantly (P <0.05), eventually disappeared completely, G1 phase significantly increased with time and concentration (P <0. 05), finaHy reached 90. 0%; the activity of cyclin D1 significantly declined (P < 0. 05), while the activity of p53 had no significant change (P > 0. 05). The apoptotic rate of MCF-7 ceHs significantly increased with the extension of time (P < 0. 05). At 6h, 12ha d2h of action time, the apoptotic rate of MCF-7 ceüs significantly increased with the increase in volatile oil concentration (P <0. 05). Morphological observation showed that the apoptosis of MCF-7 ceHs was obvious. [Conclusions] The volatile oils of Citrisarcodactylis fructus have obvious inhibition of proliferation of MCF-7 ceHs and function of inducing apoptosis, and the effects took on the dose and time dependent. 5 - 50 μoHL volatile oil of Sichuan Citrisarcodactylis fructus can inhibit the proliferation of MCF-7 ceHs and induce the apoptosis of MCF-7 ceHs through inhibiting the cyclin D1.
- Subjects
ESSENTIAL oils; BREAST cancer treatment; CANCER cell enzymes; MEDICINAL plants; CELLULAR pathology; CELL proliferation
- Publication
Medicinal Plant, 2017, Vol 8, Issue 3, p14
- ISSN
2152-3924
- Publication type
Article