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- Title
Stability-indicating Method of Specnuezhenide in Bulk Drug and Its Kinetics and Mechanisms of Degradation in Aqueous Solutions.
- Authors
WANG, Feifei; JIN, Xiuli; HUANG, Huanhuan; LIU, Tingting; ZHU, Yan; GAO, Xiumei; REN, Xiaoliang; WANG, Meng
- Abstract
The stability of specnuezhenide (SPN) extracted from FRUCTUS LIGUSTRI LUCIDI (a commonly used official Chinese herbal) was investigated by using reversed-phase high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) with a triple quadrupole (QQQ) mass detector (UPLC-MS-MS). SPN was subjected to forced decomposition under the alkaline, acidic, thermal, irradiation and oxidation conditions. The hydrolytic kinetics was calculated. Maximum stability of SPN was found at pH 7 (t0.5 - 288. 81 d). SPN was unstable in acidic, alkaline and irradiation conditions. The activation energy (Ea) of SPN degradation in aqueous solution at pH 7 (most frequently applied condition) was 47.17 kj/mol. The results showed that the degradation of SPN was pH-, temperature- and irradiation-dependent and all followed first-order kinetics. Five degradation products were detected under those conditions, which were identified as salidroside, oleoside 11-methyl, oleoside dimethyl ester, nuezhenide and ligustroside. The possible hydrolytic pathway of SPN was proposed as epimerization reaction under irradiation, meanwhile hydrolysis and methylation in acidic, alkaline and thermal conditions. The stability-indicating method was established and validated by simultaneous determination of SPN and five degradation products in bulk drug.
- Subjects
PLANT extracts; CHEMICAL decomposition; ACTIVATION energy; EPIMERIZATION; METHYLATION; HIGH performance liquid chromatography
- Publication
Medicinal Plant, 2014, Vol 5, Issue 11/12, p13
- ISSN
2152-3924
- Publication type
Article