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- Title
A sensitive enzymatic method (SK-013) for detection and quantificationof specific periodontopathogens.
- Authors
Ishihara, Kazuyuki; Naito, Yuko; Kato, Tetsuo; Takazoe, Ichiro; Okuda, Katsuji; Eguchi, Toru; Nakashima, Koichi; Matsuda, Naoki; Yamasami, Kyoko; Hasegawa, Kenji; Suido, Hirohisa; Sugihara, Kunio
- Abstract
<em>Porphyromonas gingivalis, Bacteroides forsythus</em>, and <em>Treponema denticola</em> have been found to predominate in peridontal pockets of patients with adult periodontitis. These microorganisms hydrolyze the synthetic peptide N-benezoyl-DL-arginine-2-napththylamide (BANA). In this study, we developed an enzymatic method , designated SK-013, to detect the existence of these microorganisms in subgingival plaque bacteria. This enzymatic method was based on the observation of the hydrolysis of N-carbobenzoxy-glycyl-glycyl-arginyl-3,5-dibromo-4-hydroxyaniline (N-CBz-GLy-ARg-DBHA) and made more sensitive by adding an enhancing system. The SK-013 was specifically positive for <em>P. gingivalis, B. forsythus. T. denticola</em>, and some strains of <em>Capnocytophaga</em> species, but was not specific for any of the other bacterial strains tested. This SK-013 system may be valuable for detection and quantification of periodontal disease-associated bacteria in sub-gingival plaque and thus for diagnosis of peridontal infections.
- Subjects
ENZYME kinetics; PORPHYROMONAS gingivalis; MICROORGANISMS; SYMPTOMS; PERIODONTITIS; PERIODONTAL disease
- Publication
Journal of Periodontal Research, 1992, Vol 27, Issue 2, p81
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.1992.tb01807.x