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- Title
Characterization of a Bifunctional <i>O</i>- and <i>N</i>-Glucosyltransferase from <i>Vitis vinifera</i> in Glucosylating Phenolic Compounds and 3,4-dichloroaniline in <i>Pichia pastoris</i> and <i>Arabidopsis thaliana</i>.
- Authors
Xu, Zhi-Sheng; Xue, Wei; Xiong, Ai-Sheng; Lin, Ya-Qiu; Xu, Jing; Zhu, Bo; Zhao, Wei; Peng, Ri-He; Yao, Quan-Hong
- Abstract
2,4,5-Trichlorophenol, 2,6-dimethylphenol, 3-methylcatechol, phenol, hydroquinone, catechol, and 3,4-dichloroaniline are present in the environment and are risky to humans and animals because of their wide applications in many industries. In this study, a putative uridine diphosphate glucose-dependent glycosyltransferase from Vitis vinifera (VvUGT72B1) displayed high O-glucosyltransferase or N-glucosyltransferase activity toward all these xenbiotics and was able to enhance the resistance of P. pastoris to them. Compared with wild-type Arabidopsis plants, VvUGT72B1-transgenic Arabidopsis plants showed higher resistance to all the xenobiotics except for phenol and exhibited higher removal efficiencies against all xenobiotics. Glucosides of 3-methylcatechol, 2,6-dimethylphenol, phenol, and 3,4-dichloroaniline were exported to the surrounding media by Arabidopsis plants while transgenic Arabidopsis plants exported more glucosides than wild-type Arabidopsis plants. Our findings have the potential to provide a broader spectrum remediation strategy for the phytoremoval and degradation of phenolic compounds and 3,4-dichloroaniline than previous works.
- Subjects
GLYCOSYLTRANSFERASES; TRICHLOROPHENOL; GUAIACOL; HYDROQUINONE; PICHIA pastoris; ARABIDOPSIS thaliana; GLUCOSIDES
- Publication
PLoS ONE, 2013, Vol 8, Issue 11, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0080449