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- Title
IL-1 beta, IFN gamma, IL-10 EXPRESSION IN PLACENTAL TISSUES OF PATIENTS WITH RHEUMATOID ARTHRITIS AND SYSTEMIC LUPUS ERYTHEMATOSUS.
- Authors
G., Martínez-Bonilla; J. F., Muñoz-Valle; G., Orozco-Barocio; G., Bernard-Medina; A. M., Gutiérrez-Chavarin; A., Daneri-Navarro; V., Delgado-Rizo; J., Armendáriz-Borunda; M., Vázquez-Del Mercado
- Abstract
Rheumatoid arthritis (RA) and systemic lupus erithematosus (SLE) are autoimmune diseases. Patients with RA during pregnancy tends to amelioration, whereas patients with SLE tends to exacerbate in the clinical outcome. Different cytokines have been identified in placental tissue during gestation in diverse diseases. However, the role of cytokines in rheumatic diseases has not been fully clarified. Objective: To investigate mRNA expression of IL-1β, IFNγ, and IL-10 in placental tissue at term of patients with RA, SLE and also in healthy pregnant women. Patients and Methods: We recruited 20 placentas which corresponded to the following groups: 7 RA, 6 SLE pregnant patients and 7 healthy pregnant women. Cytokine mRNA expression was determined by semiquantitative PCR using GAPDH as a constitutive gene. The amplified fragment corresponding to IL-1β, IFNγ, IL-10 and GAPDH were: 288, 477, 351 and 566 bp, respectively. Statistical analysis was performed using SPSS version 10.0. Results: IFNγ mRNA expression levels increased in RA placental tissues (458(200 RAU), in comparison with SLE patients (126.5(0.7 RAU) (p<0.05). High levels of IFNγ in placental tissues of healthy women (539(27 RAU1 were observed in comparison with SLE patients (p<0.05). IL-1β mRNA expression level showed no significant difference between the studied groups. With regard to IL-10 expression, this cytokine was not observed in any placental tissue studied. Conclusions: IL-1β mRNA expression level showed no significant difference between the studied groups. In addition, expression of IL-10 was not observed in any of the studied groups. This immunological pattern may be attributable to important processes underlying parturition itself, instead to the rheumatic diseases.
- Subjects
INTERLEUKIN-1; INTERLEUKIN-10; RHEUMATOID arthritis; SYSTEMIC lupus erythematosus; AUTOIMMUNE diseases; CYTOKINES; MESSENGER RNA; RHEUMATISM; PATIENTS
- Publication
Revista VacciMonitor (Vacunología y Temas Afines), 2002, Vol 11, Issue 4, p1
- ISSN
1025-028X
- Publication type
Article