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- Title
Upregulation of T-Cell-Stimulating Activity of Mycobacteria-Infected Macrophages.
- Authors
Kimura, H.; Maeda, Y.; Takeshita, F.; Takaoka, L. E.; Matsuoka, M.; Makino, M.
- Abstract
Macrophages are one of the most abundant host cells to come in contact with mycobacteria. However, the infected macrophages less efficiently stimulate autologous T cells in vitro. We investigated the effect of the induction of phenotypic change of macrophages on the host cell activities by using Mycobacterium leprae as a pathogen. The treatment of macrophages with interferon-γ (IFN-γ), GM-CSF and interleukin-4 deprived macrophages of CD14 antigen expression but instead provided them with CD1a, CD83 and enhanced CD86 antigen expression. These phenotypic features resembled those of monocyte-derived dendritic cells (DC). These macrophage-derived DC-like cells (MACDC) stimulated autologous CD4+ and CD8+ T cells when infected with M. leprae. Further enhancement of the antigen-presenting function and CD1a expression of macrophages was observed when treated with IFN-γ. The M. leprae-infected and -treated macrophages expressed bacterial cell membrane-derived antigens on the surface and were efficiently cytolysed by the cell membrane antigen-specific CD8+ cytotoxic T lymphocytes (CTL). These results suggest that the induction of phenotypic changes in macrophages can lead to the upregulation of host defence activity against M. leprae.
- Subjects
MYCOBACTERIUM leprae; MACROPHAGES; T cells; MYCOBACTERIUM; INTERLEUKIN-4; ANTIVIRAL agents
- Publication
Scandinavian Journal of Immunology, 2004, Vol 60, Issue 3, p278
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.0300-9475.2004.01472.x