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- Title
Proliferation, migration and apoptosis of periodontal ligament cells after tooth replantation.
- Authors
Sato, K; Muramatsu, T; Tsuchiya, Y; Masaoka, T; Enokiya, Y; Hashimoto, S; Shimono, M
- Abstract
Oral Diseases (2010) 16, 263–268 Objective: The aim of this study was to investigate the proliferation, migration and death of periodontal ligament (PDL) cells after tooth replantation. Materials and methods: Maxillary first molars were extracted from 4-week-old male ( n = 28) Sprague–Dawley rats and immediately replanted, after which, proliferation, migration and death of PDL cells were investigated. Results: At 3 days after tooth replantation, many proliferative cell nuclear antigen (PCNA)-positive PDL cells were observed on the alveolar bone side, but fewer on the root side. However, while a gradual decrease was observed in number of PCNA-positive PDL cells on the alveolar bone side until 7 days, an increase was seen on the root side. At 3 weeks, cells labeled with PKH26 (fluorescent dye into plasma membrane) were located in the middle of the PDL space. However, these PKH26-labeled cells did not spread to the surface of the cementum or the alveolar bone. TUNEL-positive cells were observed on both the bone and root sides at 3 days. Number of apoptotic cells increased until 7 days on the bone sides, but decreased on root sides. Conclusion: These results suggest that both cell proliferation and apoptosis occur in different patterns and at different times to maintain regular spacing of the PDL after tooth replantation.
- Subjects
PERIODONTAL ligament; CELL death; ALVEOLAR process; QUANTITATIVE research; CEMENTUM; TOOTH replantation
- Publication
Oral Diseases, 2010, Vol 16, Issue 3, p263
- ISSN
1354-523X
- Publication type
Article
- DOI
10.1111/j.1601-0825.2009.01594.x