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- Title
DETECTION OF AQUAPORIN 7 (AQP7) GENE FRAGMENT FROM RATTUS ARGENTIVENTER (RICE-FIELD RAT) TESTES.
- Authors
Ismail, Is'adah Khisma; Cheah Yoke Kqueen; Lee Learn Han; Sukardi, Sabrina
- Abstract
Abstract : Aquaporin is a water channel that forms as an integral membrane protein serving the permeation of water molecules through the membrane. Aquaporin exists in the plasma membrane as homotetramers. Besides facilitating the transport of water, in some cases, other small solutes such as glycerol are also transported across the membrane through aquaporin. Aquaporin 7 (AQP7) is an aquaglyceroporin that is permeable to water, glycerol and urea. AQP7 is expressed in plasma membranes of adipose tissue, testis and kidney. The ontogeny and distribution of AQP7 in rat testis suggest involvement of major physiologic changes of testis development and spermatogenesis. In this study, we aim to amplify the AQP7 from Rattus argentiventer (rice-field rat) testes using one specific set of primer. AQP7 from 3 different rat's testes were successfully amplified using Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR) technique. The amplified 810bp fragments derived from our optimization is then sequenced and compared to the various AQP7 sequences available from the NCBI database. Rattus argentiventer is the most important pest in paddy fields in tropical Asian countries. Rice farmers lost more than 8% of their rice crop each season due to these rats. Discovery of highly conserved AQP7 among these pests indirectly leads to the possibility of curbing the increase in rat populations. It may also serve as an alternative in biocontrolling of these rats. This will indirectly control rats' populations in paddy areas, reducing crop losses and also in the end increasing farmers' income.
- Subjects
AQUAPORINS; MEMBRANE proteins; SPERMATOGENESIS; POLYMERASE chain reaction; LABORATORY rats
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, p132
- ISSN
1394-195X
- Publication type
Article