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- Title
A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system.
- Authors
Huang, Li-Li; Jin, Yong-Jie; Zhao, Dongxu; Yu, Chao; Hao, Jian; Xie, Hai-Yan
- Abstract
The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification. [Figure not available: see fulltext.]
- Subjects
SIALIC acids; BIOCOMPATIBILITY; BORONIC acids; BIOCONJUGATES; QUANTUM dots; VESICULAR stomatitis; FLUORESCENCE
- Publication
Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 11, p2687
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-014-7651-9